| Protein name |
Brazzein |
| Host compatibility |
Komagatella phaffi (Pichia pastoris) |
| Yield |
0.639 g/L (Rong et al., 2025)
|
| Scale (in specific example) |
Lab scale in bioreactor (Rong et al., 2025)
|
| Biggest scale product has been develloped in this organism |
Perfect Day has recieved GRAS notice for the production of brazzein in K. phaffi, indicating plans to produce at pilot or industrial scale
|
| Regulatory status in Europe |
Not allowed |
| Regulatory status in other parts of the world |
FDA GRAS approval in the US. Not allowed in Canada
|
| Companies producing this protein in this organism |
|
| Extra/remark |
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| Publications/references |
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Rong, W., Wang, X., Shi, Z., Liu, L., Zhang, Z., Fang, Y., Sun, J., Lin, L., & Wang, G. (2025). Systematic Engineering of Komagataella phaffii for Efficient Production of Sweet‐Tasting Protein Brazzein. Biotechnology Journal, 20(6). https://doi.org/10.1002/biot.70046
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Saraiva, A., Carrascosa, C., Ramos, F., Raheem, D., Pedreiro, S., Vega, A., & Raposo, A. (2023). Brazzein and Monellin: Chemical Analysis, Food Industry Applications, Safety and Quality Control, Nutritional Profile and Health Impacts. Foods, 12(10), 1943. https://doi.org/10.3390/foods12101943
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Novik, T. S., Koveshnikova, E. I., Kotlobay, A. A., Sycheva, L. P., Kurochkina, K. G., Averina, O. A., Belopolskaya, M. V., Sergiev, P. V., Dontsova, O. A., Lazarev, V. N., Maev, I. V., Kostyaeva, M. G., Eremeev, A. V., Chukina, S. I., & Lagarkova, M. A. (2023). Sweet-Tasting Natural Proteins Brazzein and Monellin: Safe Sugar Substitutes for the Food Industry. Foods, 12(22), 4065. https://doi.org/10.3390/foods12224065
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Hellekant, G. (2005). Brazzein a Small, Sweet Protein: Discovery and Physiological Overview. Chemical Senses, 30(Supplement 1), i88–i89. https://doi.org/10.1093/chemse/bjh127
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Levprot Bioscience. (n.d.). Brazzein: The ultimate sugar alternative with Precision Fermentation - Levprot Bioscience. Retrieved September 3, 2025, from https://levprot.com/brazzein-the-ultimate-sugar-alternative-with-precision-fermentation/
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Carlson, A., Armentrout, R. W., Ellis, T. P., & Llp, T. a. L. N. A. (2014, June 3). US20100112639A1 - Enhanced production and purification of a natural high intensity sweetener - Google Patents. Retrieved September 3, 2025, from https://patents.google.com/patent/US20100112639A1/en
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Farag, M. A., Rezk, M. M., Elashal, M. H., El-Araby, M., Khalifa, S. a. M., & El-Seedi, H. R. (2022). An updated multifaceted overview of sweet proteins and dipeptides as sugar substitutes; the chemistry, health benefits, gut interactions, and safety. Food Research International, 162, 111853. https://doi.org/10.1016/j.foodres.2022.111853
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